Anti-Cdc42 Antibody

Our Cdc42 mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Cdc42 and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.

Short Description

Our Cdc42 mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Cdc42 and is antigen affinity purified. It is great for use in WB, ICC.

Synonyms

CDC-42, G25K

Gene Name

CDC42

UniProt

Q8CFN2

Reactivity

Human, Mouse, and Rat

Immunogen

Clone (M430) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.

Target

Cdc42

Clonality

Monoclonal

Clone

M430

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Signaling, Kinase Signaling

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:500

Specificity

The antibody detects a 21 kDa* protein in human Jurkat cells, rat A7r5 cells, and mouse brain. The antibody also detects recombinant full length rat Cdc42 fusion protein, but does not recognize a full length human RhoA GST fusion protein.