Anti-CD99 (Extracellular region) Antibody

Our CD99 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CD99 (Extracellular region) and is protein g purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

The glycosylated transmembrane protein CD99 is involved in many essential cellular functions including cell adhesion, migration, cell death, differentiation, and intracellular protein trafficking. The CD99 gene encodes two distinct proteins, type I is 32 kDa and type II is 28 kDa, which are a result of the alternative splicing of the cytoplasmic region. These CD99 isoforms are expressed in a cell-type-specific manner and may have distinct functions. CD99 is overexpressed in several types of sarcomas, lymphomas, gliomas, neuroendocrine tumors, and some breast cancers. In these tumors, CD99 may have oncogenetic functions that promote migration, invasion, and metastasis of tumor cells. However, other neoplasms, carcinomas, and sarcomas have CD99 expression in benign or early-stage tumors, but lower expression in the advanced-stage counterparts. In these tumors, CD99 may have oncosuppressor signaling, and its re-expression can lead to the reversal of malignancy. Thus, CD99 is an important membrane protein involved in many aspects of cell migration and adhesion in normal and diseased cells.

Short Description

Our CD99 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CD99 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Synonyms

CD99 antigen, 12E7, E2 antigen, MIC2 T-cell surface glycoprotein E2, MIC2X, MIC2Y

Gene Name

CD99

UniProt

P14209

Reactivity

Human, Mouse, and Rat

Immunogen

Clone M030 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.

Target

CD99 (Extracellular region)

Clonality

Monoclonal

Clone

M030

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Cell Adhesion Molecules, Immunology, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

30-32

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:500

IP Dilution

1:100

Specificity

Clone M030 detects 30 and 32 kDa* bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation. In addition, the antibody labels live, unfixed MeWo cells.