Anti-CD46 (Extracellular region) Antibody

Our CD46 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD46 (Extracellular region) and is protein g purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

CD46 is a complement regulatory protein that is also called membrane cofactor protein. This protein is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 exhibits a cofactor activity that promotes inactivation of C3b and C4b by serum factor 1, thereby protecting host cells from complement-dependent cytotoxicity. CD46 can also function as a receptor for selected bacteria and viruses, and is reportedly required for proper fusion of spermatozoa to the oocyte membrane during fertilization. CD46 is overexpressed in medulloblastoma tumors, and CD46 expression has been linked with poor prognosis in breast cancer. The upregulation of CD46 may protect cancer cells from complement-dependent cytotoxicity to facilitate cancer cell immune evasion.

Short Description

Our CD46 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD46 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Synonyms

Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, CD46, MCP, MIC10

Gene Name

CD46

UniProt

P15529

Reactivity

Human

Immunogen

Clone (M037) was generated from a proprietary antigen related to native human CD46 expressed in NCI-H1915 lung cancer cell line.

Target

CD46 (Extracellular region)

Clonality

Monoclonal

Clone

M037

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Cell Adhesion Molecules, Immunology, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

45-60

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:100

Specificity

Clone M037 mouse monoclonal antibody detects a 45-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human lung tissues. This antibody weakly detects denatured CD46. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.