Anti-CD44 (Hyaluron Binding Region) Antibody

Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.

Our CD44 (Hyaluron Binding Region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD44 (Hyaluron Binding Region) and is Protein G purified. It is great for use in WB, ICC, IP.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Clone M588 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of native MDA-MB-231, A431, and A549 cell lysates. The antibody also detects denatured CD44 but with lower affinity. In addition, Clone M588 binds the native form of a recombinant human CD44 protein that contains only the hyaluron binding region. The antibody can be used in multiple applications including western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well as for detecting CD44 in live, unfixed cells.