Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody

Our δ1-Catenin (Tyr-228) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat δ1-Catenin (Tyr-228) and is protein a purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

Short Description

Our δ1-Catenin (Tyr-228) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat δ1-Catenin (Tyr-228) and is Protein A purified. It is great for use in WB, ICC.

Synonyms

Pp120 Src substrate, p120

Gene Name

CTNND1

UniProt

O60716

Modification Site

Tyr-228

Reactivity

Human, Mouse, and Rat

Immunogen

Clone M356 was generated from a phospho-δ1-Catenin (Tyr-228) synthetic peptide containing amino acid residues around tyrosine 228 of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin.

Target

δ1-Catenin (Tyr-228)

Clonality

Monoclonal

Clone

M356

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Adhesion Molecules, Cell Signaling, Wnt Signaling, Immunology, Oncoproteins, Transcription Regulation

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb/Phosphorylated

Molecular Weight

110

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:1000

Specificity

The antibody detects a 110 kDa* protein corresponding to the molecular mass of δ1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells treated with pervanadate. This antibody does not detect this band after alkaline phosphatase treatment.