Anti-Unphosphorylated N-Cadherin (Tyr-820) Antibody

Our Unphosphorylated N-Cadherin (Tyr-820) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Unphosphorylated N-Cadherin (Tyr-820) and is antigen affinity purified. It is great for use in ELISA, WB.

Product Specifications

Background

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Short Description

Synonyms

Cadherin-2, Neural-Cadherin, CD325

Gene Name

CDH2

UniProt

P19022

Reactivity

Human, Mouse, and Rat

Immunogen

Unphosphorylated N-Cadherin (Tyr-820) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved region in R-cadherin.

Target

Unphosphorylated N-Cadherin (Tyr-820)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

Cell Adhesion Molecules, Cell Markers, Extracellular Matrix Proteins, Oncoproteins

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

130

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

This antibody was cross-adsorbed to phospho-N-cadherin (Tyr-820) peptide before affinity purification using unphosphorylated N-cadherin (Tyr-820) peptide. The purified antibody detects a strong band at 130 kDa* in western blots of serum-starved human endothelial cells, but is decreased in pervanadate treated cells. This reactivity in western blots is blocked specifically by unphosphorylated N-cadherin (Tyr-820) peptide.