Anti-B7-H3/CD276 (Extracellular region) Antibody

Our B7-H3/CD276 (Extracellular region) Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human B7-H3/CD276 (Extracellular region) Antibody and is protein g purified. It is great for use in ELISA, WB, IP, ICC.

Product Specifications

Background

B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 is a membrane protein with an extracellular region that includes two Ig-like V-type domains and two IgG-like C2-type domains, and a short intracellular domain. B7-H3 is a regulatory molecule for immune reactions, such as T cell proliferation and IFN-γ production. In colon cancers, B7-H3 inhibits T-cell cytotoxicity, and blocking B7-H3 function enhances T-cell cytotoxicity toward cancer cells. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. In cancer, B7-H3 is expressed in various tumor types including prostate, breast, colon, lung, and gastric cancers. The B7-H3 expression level correlates with tumor growth, invasion, metastasis, malignant stage, and recurrence rate. The inhibition or blockade of B7-H3 function could be an important immunotherapeutic approach for several types of cancer

Short Description

Our B7-H3/CD276 (Extracellular region) Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human B7-H3/CD276 (Extracellular region) Antibody and is Protein G purified. It is great for use in WB, IP, ICC.

Synonyms

CD276, B7-H3, B7H3, B7RP-2, B7 homolog 3

Gene Name

CD276

UniProt

Q5ZPR3

Reactivity

Human

Immunogen

Clone (M045) was generated from a recombinant protein that included the extracellular region of human B7-H3 protein.

Target

B7-H3/CD276 (Extracellular region) Antibody

Clonality

Monoclonal

Clone

M045

Conjugation

Unconjugated

Applications

ELISA, WB, IP, ICC

Field of Research

Immunology

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

100-120

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:100

Specificity

Clone M045 mouse monoclonal antibody detects a 100-120 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinomas. The antibody detects B7-H3 in membranes and cytoplasm in MCF7 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.