Anti-Annexin A1 Antibody

Our Annexin A1 mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Annexin A1 and is protein g purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13) . These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.

Short Description

Our Annexin A1 mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Annexin A1 and is Protein G purified. It is great for use in WB, ICC, IP.

Synonyms

Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I1, Phospholipase A2 inhibitory protein p35, ANXA1, ANX1, LPC1

Gene Name

ANXA1

UniProt

P04083

Reactivity

Human

Immunogen

Clone M021 was generated from a proprietary antigen related to human Annexin A1 in the A549 lung carcinoma cell line.

Target

Annexin A1

Clonality

Monoclonal

Clone

M021

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Apoptosis, Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation, Extracellular Matrix Proteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:100

Specificity

Clone M021 detects a 38 kDa* protein corresponding to the molecular mass of Annexin A1 on SDS-PAGE immunoblots of human A431, MDA-MB-231, and A549. The antibody also detects full length recombinant human Annexin A1, but not Annexin A2 or Annexin A5 recombinant proteins. The antibody works for western blot, ELISA, immunocytochemical labeling, and immunoprecipitation.