Anti-Akt (Ser-473), Phosphospecific Antibody

Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.

Our Akt (Ser-473) phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Akt (Ser-473) and is Protein G purified. It is great for use in WB, IP.

Clone M114 was generated from a peptide containing amino acid residues surrounding Serine 473 in human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Akt on SDS-PAGE immunoblots of mouse NIH3T3 cells treated with PDGF and human A431 cells treated with EGF.