Lineage Cocktail Assay kit (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC Conjugated

USE: The Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC consists of the mixture of antibodies which stain human lymphocytes, monocytes, eosinophils and neutrophils. Peripheral blood dendritic cells and basophils can be identified by lack of staining with this reagent. Principle: This test is based on specific binding of monoclonal antibodies to the antigenic determinants expressed on the surface of human lymphocytes, monocytes, eosinophils and neutrophils in peripheral blood. Dendritic cells and basophils can be identified in the peripheral blood by their lack of staining with this Lineage Cocktail using common flow cytometer device equipped with 488 nm laser. The monoclonal antibodies used in the Lineage Cocktail are labeled with Fluorescein isothiocyanate (FITC) which is excited by single laser beam from a flow cytometer during analysis. Staining of human blood cells is performed by incubation of blood samples with the Lineage Cocktail and, whenever applicable, the cells can be simultaneously stained by other monoclonal antibodies conjugated to appropriate fluorochromes. For example, dendritic cells can be distinguished from basophils by positive staining with PEconjugated anti-HLA-DR antibody. Staining whole peripheral blood with mixture of conjugated antibodies is followed by a lysis of red blood cells, wash and subsequent flow cytometry analysis. Specificity: The monoclonal antibody MEM-57 reacts with CD3 complex, expressed on all peripheral blood T lymphocytes. The antibody MEM-15 reacts with CD14, expressed on monocytes, macrophages and weakly on neutrophils. The antibody LNK16 reacts with CD16, expressed on NK-cells, monocytes, macrophages and neutrophils. The antibody LT19 reacts with CD19, expressed on B lymphocytes. The antibody LT20 reacts with CD20, expressed on B lymphocytes. The antibody MEM-188 reacts with CD56, expressed on NK lymphocytes.