Simeprevir (sodium salt)

A stock solution may be made by dissolving the simeprevir (sodium salt) in the solvent of choice. Simeprevir (sodium salt) is soluble in organic solvents such as DMSO and dimethyl formamide, which should be purged with an inert gas. The solubility of simeprevir (sodium salt) in these solvents is approximately 30 mg/ml. Simeprevir (sodium salt) is also slightly soluble in ethanol. Simeprevir (sodium salt) is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, simeprevir (sodium salt) should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. Simeprevir (sodium salt) has a solubility of approximately 0.33 mg/ml in a 1:2 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.