Human AFP (Alpha Fetoprotein) Pre-Coated ELISA Kit

This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate. Standards and samples are pipetted into the wells and AFP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AFP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AFP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.