Human EGFR / HER1 / ErbB1 Recombinant Protein (Fc Tag)

Source : HEK293 Cells As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-alpha, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT) . In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Product Specifications

Product Name Alternative

ERBB Protein, ERBB1 Protein, HER1 Protein, mENA Protein, NISBD2 Protein, PIG61 Protein

Reactivity

Human

Purification

> 97 % as determined by SDS-PAGE

Components

Formulation Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped o

Storage Conditions

Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Applications Notes

Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 10 μg/ml (100 μL/well) can bind human EGFR with a linear range of 0.64-400 ng/ml. Endotoxin :< 1.0 EU per μg of the protein as determined by the LAL method. Other pack size also available.