TNF-alpha, Soluble (human) (Recombinant)

A FLAG®-tag is fused at the N-terminus of human TNF-alpha (aa 85-223) .Specific Binds to human and mouse TNF-R1.Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (MultimericTNF-alpha (32-190048) . Since the MultimericTNF-alpha mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either normal" TNF-alpha or Multimeric TNF-alpha can be used."

Product Specifications

Product Name Alternative

Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin

Reactivity

Human

Applications

Functional Assay

Purification

>=95% (SDS-PAGE)

Components

10 μg size: Reconstitute with 100 μl sterile water. 50 μg size: Reconstitute with 50 μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.10 μg size: 0.1 mg/mL after reconstitution. 50 μg size: 1 mg/mL after reconstitution. Lyophilized. Contains PBS.

Storage Conditions

Short Term Storage +4°C ; Long Term Storage-20°C ; After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0. 1% BSA should be used for further dilutions. Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Applications Notes

MW :~17kDa (SDS-PAGE) Biological Activity: Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells) . Endotoxin Content: <0.01EU/μg purified protein (LAL test; Lonza) .