IL-10 (human) :Fc (human) (Recombinant) (non-lytic)

Source :CHO cells Specific Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggest the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.Other Product Data Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC) .

Product Specifications

Product Name Alternative

Interleukin-10; Cytokine Sythesis Inhibitory Factor; CSIF

Reactivity

Human

Applications

Functional Assay

Purification

>=98% (SDS-PAGE)

Components

Reconstitute 10μg vial in 100μl sterile water. Reconstitute 50μg vial in 50μl sterile water. Add 1X PBS to the desired protein concentration. Lyophilized from 0.2μm-filtered solution in PBS.

Storage Conditions

Short Term Storage +4°C ; Long Term Storage-20°C ; Avoid freeze/thaw cycles. Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Applications Notes

Biological Activity: Shows the biological function of the IL-10 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain. Endotoxin Content: <0.06EU/μg protein (LAL test; Lonza) .