Recombinant SARS-CoV-2 S1+S2 ECD (S-ECD) Protein His tag

Source: HEK293 cells. Endotoxin < 0.1 EU/μg of the protein by LAL method. Recombinant 2019-nCoV S1+S2 ECDÃ ̄1⁄4Ë†S-ECDÃ ̄1⁄4â€° Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence Cys15-Gln1208 (Pro986, 987) (682-685GSAS) of 2019-ncov S1+S2 ECDÃ ̄1⁄4Ë†S-ECDÃ ̄1⁄4â€° (Accession #QHD43416.1) fused with a 6XHis tag at the C-terminus.

Product Specifications

Product Name Alternative

Cov spike Protein; 2019-nCoV; ncov spike Protein; 2019-nCoV; coronavirus spike Protein; 2019-nCoV; S glycoprotein; COVID-19; S protein; Spike glycoprotein

Gene ID

43740568

Applications

Functional Assay

Purification

>97% by SDS-PAGE.

Components

Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

Storage Conditions

This product is stable at -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Applications Notes

Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike S1+S2 ECD at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-3.03 ng/mL.

Amino Acids

The target protein is expressed with sequence Cys15-Gln1208 (Pro986, 987) (682-685GSAS) of 2019-ncov S1+S2 ECDÃ ̄1⁄4Ë†S-ECDÃ ̄1⁄4â€° (Accession #QHD43416.1) fused with a 6XHis tag at the C-terminus.