Recombinant Human ACE2 Protein with His and Avi tag

Source: HEK293 cells. Endotoxin: < 0.1 EU/μg of the protein by LAL method. Calculated MW : 86.2kDa Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a 6XHis-Avi tag at the C-terminus.

Product Specifications

Product Name Alternative

ACE-2; ACEH; ACE2

Gene ID

59272

UniProt

Q9BYF1

Applications

Functional Assay

Purification

>95% by SDS-PAGE.

Components

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4. Reconstitution: For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Storage Conditions

Store the lyophilized protein at -20°C to -80 °C for long term.After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. This product is stable at-70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Applications Notes

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2-His at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike S1 with a linear range of 1.5-104.5ng/mL.

Amino Acids

Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a 6XHisÃ ̄1⁄4Å’Avi tag at the C-terminus.