Recombinant SARS-CoV-2 Spike S1 Protein His tag

Source: HEK293 cells. Endotoxin: < 0.1 EU/μg of the protein by LAL method. Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.

Product Specifications

Product Name Alternative

S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein

Gene ID

43740568

Applications

Functional Assay

Purification

> 90% by SDS-PAGE.

Components

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

Storage Conditions

Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. This product is stable at-70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Applications Notes

Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike S1-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-36.5 ng/mL.|Immobilized Human ACE2 on COOH Chip, can bind 2019-nCoV Spike S1 with an affinity constant of 27.3 nM as determined in a SPR assay (Nicoya OpenSPR) .

Amino Acids

The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.