Recombinant SARS-CoV-2 Spike RBD Protein, mFc tag

Source: HEK293 cells. Recombinant 2019-nCoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus.

Product Specifications

Product Name Alternative

S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein

Gene ID

43740568

Applications

Functional Assay

Purification

>95% by SDS-PAGE.

Components

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

Storage Conditions

Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. This product is stable at-70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Applications Notes

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 Protein at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike RBD-mFc ProteinÃ ̄1⁄4Å’the EC50 of Recombinant 2019-nCoV Spike RBD-mFc Protein is 2.58 ng/mL. Endotoxin: < 0.1 EU/μg of the protein by LAL method.

Amino Acids

The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus.