Recombinant Sars-Cov-2 (COVID-19/2019-nCov) Spike S1 Protein
Source: HEK293 cells. Endotoxin < 1.0 EU/μg of the protein by LAL method. Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6XHis tag at the C-terminus.
Product Specifications
Product Name Alternative
S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, novel coronavirus s1 Protein
Gene ID
43740568
Applications
Functional Assay
Purification
>95% by SDS-PAGE.
Components
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions
Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Applications Notes
Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL.
Amino Acids
Tag Fc, 6XHis tag at the C-terminus. (Val11-Arg682)
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