Cynomolgus ALPP Protein, His Tag

Description :Recombinant Cynomolgus ALPP protein with C-terminal 10Ãƒâ€”His tagBackground: The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Aug 2020]Description: Recombinant Cynomolgus ALPP protein with C-terminal 10Ãƒâ€”His tagMolecular Characterization: ALPP (Ile21-Asp504) 10Ãƒâ€”His tagMolecular Weight :The protein has a predicted molecular mass of 54.1 kDa after removal of the signal peptide. The apparent molecular mass of cALPP-His is approximately 55-70 kDa due to glycosylation.Tag :C-10Ãƒâ€”His tag

Product Specifications

Product Name Alternative

ALP; IAP; ALPI; PALP; PLAP; PLAP-1

UniProt

XP_045223825.1

Host

HEK293

Target Antigen

ALPP

Purification

The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

Components

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Storage Conditions

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing) . Lyophilized proteins are shipped at ambient temperature.