Human MET Protein, His Tag

Description :Recombinant human MET Protein with C-terminal 10Ãƒâ€”His tagBackground : This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. Molecular Characterization: mass of 103.0 kDa after removal of the signal peptide. The apparent molecular mass of MET-His is approximately 70-130 kDa due to glycosylation.Tag :C-10Ãƒâ€”His tag

Product Specifications

Product Name Alternative

DA11; HGFR; AUTS9; RCCP2; c-Met; DFNB97

UniProt

P08581

Host

HEK293

Target Antigen

MET

Purification

The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

Components

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Storage Conditions

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing) . Lyophilized proteins are shipped at ambient temperature.