Human CGRPR and RAMP1 Heterodimer Protein, hFc Tag and mFc Tag

Description :Recombinant human CGRPR protein with C-terminal human Fc tag and human RAMP1 protein with C-terminal mouse Fc tagBackground : The CGRP receptor (CGRPR) is a member of family B G protein coupled receptors (GPCRs), is expressed throughout the trigeminal system, including neurons and endothelial cells. They usually function with accessory proteins such as receptor activity modifying proteins (RAMPs) and Na/H exchange regulatory factors (NHERFs) . CGRPR is a heterodimer complex of the calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein 1 (RAMP1) . Therapeutics for migraine treatment are mostly targeting CRLR-RAMP1 protein-protein interaction surfaces, thereby blocking CGRP activity.Molecular Characterization: mass of 39.9 and 36.7 kDa after removal of the signal peptide. The apparent molecular mass of CGRPR-hFc and RAMP1-mFc is approximately 35-70 kDa due to glycosylation.Tag :C-Human Fc tag and C-mouse Fc tag

Product Specifications

Product Name Alternative

CRLR; CGRPR; LMPHM8 and RAMP1

UniProt

Q16602 and O60894

Host

HEK293

Target Antigen

CGRPR and RAMP1

Purification

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Components

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Storage Conditions

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing) . Lyophilized proteins are shipped at ambient temperature.