Recombinant Human SEMA4D Protein, His Tag

Molecular Characterization: SEMA4D (Met22-Arg734) 6Ã-His tagMolecular weight: The protein has a predicted molecular mass of 80 kDa after removal of the signal peptide. The apparent molecular mass of SEMA4D-His is approximately 95-130 kDa due to glycosylation.Description: Recombinant human SEMA4D protein with C-terminal6Ã-His tagCell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed: 20877282) . Regulates GABAergic synapse development (By similarity) . Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity) . Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed: 19788569) . Promotes the migration of cerebellar granule cells (PubMed: 16055703) . Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed: 8876214) . Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed: 16055703) .[UniProtKB/Swiss-Prot Function]

Product Specifications

Product Name Alternative

A8; BB18; GR3; CD100; C9orf164; CD100; SEMAJ

UniProt

Q92854

Host

HEK293

Target Antigen

SEMA4D

Components

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Storage Conditions

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing) . Lyophilized proteins are shipped at ambient temperature.