Recombinant Human NPR1 Protein, hFc Tag

Molecular Characterization: NPR1 (Gly33-Glu473) hFc (Glu99-Ala330) Molecular weight: The protein has a predicted molecular mass of 75.0 kDa after removal of the signal peptide. The apparent molecular mass of NPR1-hFc is approximately 95-130 kDa due to glycosylation.Description: Recombinant human NPR1 protein with C-terminal human Fc tagGuanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]) . The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961) . Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively) .[supplied by OMIM, May 2009]