Recombinant Human RSPO1 (21-263) Protein, His Tag

Molecular Characterization: RSPO1 (21-263) (Ser21-Ala263) 6Ã-His tagMolecular weight: The protein has a predicted molecular mass of 27.6 kDa after removal of the signal peptide. The apparent molecular mass of RSPO1 (21-263) -His is approximately 35-55 kDa due to glycosylation.Description: Recombinant human RSPO1 (21-263) protein with C-terminal6Ã-His tagThis gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.

Product Specifications

Product Name Alternative

R-spondin-1, hRspo1

UniProt

Q2MKA7

Host

HEK293

Target Antigen

RSPO1

Components

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Storage Conditions

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing) . Lyophilized proteins are shipped at ambient temperature.