IL5 Canine

Source:Sf9, Baculovirus cells.Physical Appearance:Sterile Filtered colorless solution.Biological ActivityMeasured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range = 15 ng/ml.The protein encoded by IL5is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL5is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of IL5is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF) . Interleukin-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. Interleukin-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.IL5 Canine produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 119 amino acids (22-134 aa) and having a molecular mass of 13.9kDa.IL5 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.