CYB5A Human, Sf9

Source: Sf9, Baculovirus cells.Sterile filtered colorless solution.Cytochrome b5 isoform 1 or CYB5A, is part of the cytochrome b5 family which is bound to the cell membrane. CYB5A has a key role in the stearyl-CoA-desaturase activity as it reduces ferric hemoglobin to ferrous hemoglobin. Cytochrome b5 isoform 1 performs as an electron carrier for numerous oxygenases that are imbedded in the cell membrane. CYB5A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 117 amino acids (1-108a.a.) and having a molecular mass of 13.3kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa) .CYB5A is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specifications

Product Name Alternative

Cytochrome b5 isoform 1, CYB5A, CYB5, MCB5, Microsomal cytochrome b5 type A.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

CYB5A protein solution (1mg/mL) contains phosphate buffered saline (pH7.4) and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.