Recombinant Human Interleukin-5 Receptor Subunit Alpha/IL-5 Ra (C-6His)

Source: Human Cells. MW :36.7kD. Recombinant Human Interleukin-5 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Asp21-Glu335 is expressed with a 6His tag at the C-terminus. Interleukin-5 Receptor alpha (IL-5Ra, CD125) is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology. Mature human IL-5 Ra consists of a 322 aa extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 20 aa transmembrane segment, and a 58 aa cytoplasmic domain. Within the ECD, human IL-5Ra shares 71% aa sequence identity with mouse and rat IL-5 Ra. Alternate splicing of human IL-5 Ra generates soluble secreted forms which function as IL-5 antagonists. The high affinity receptor for IL-5 is a complex that consists of the ligand binding IL-5 Ra and the transmembrane common beta chain (betac/CD131) which is shared with the receptor complexes for IL-3 and GMCSF. IL-5 Ra binds IL-5 at low affinity and then associates with preformed betac oligomers to form the signaling competent receptor complex. IL-5 stimulation of CD34+ hematopoietic progenitor cells induces the up-regulation of transmembrane IL-5Ra followed by eosinophilic differentiation and activation.