Recombinant Human a-Soluble NSF Attachment Protein/SNAP-a/NAPA (N-6His)

Source: E.coli. MW :35.4kD. Recombinant Human SNAP-alpha is produced by our E.coli expression system and the target gene encoding Met1-Arg295 is expressed with a 6His tag at the N-terminus. a-Soluble NSF Attachment Protein (SNAP-a) is a member of the SNAP (Soluble NSF Attachment Protein) family. SNAP-a interacts with PRKCABP and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2. SNAP-a is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. SNAP-a is in charge of the binding of NSF and therefore the formation of a 20S fusion particle.

Product Specifications

Gene Name

NAPA

Gene ID

8775

UniProt

P54920

Components

Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.

Shipping Conditions

The product is shipped at ambient temperature.

Storage Conditions

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Applications Notes

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Endotoxin : Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Amino Acids

MGSSHHHHHHSSGLVPRGSHMDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR