Recombinant Human DNA Polymerase beta (C-6His)

Source: E.coli. MW :39.23kD. Recombinant Human DNA Polymerase beta is produced by our E.coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus. Human DNA polymerase beta is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase beta.

Product Specifications

Gene Name

POLB

Gene ID

5423

UniProt

P06746

Components

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8.

Shipping Conditions

The product is shipped on dry ice/ice packs.

Storage Conditions

Store at -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Applications Notes

Endotoxin : Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Amino Acids

MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSEVEHHHHHH