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PLGF1 Human

Source: Escherichia coli.Sterile Filtered White lyophilized (freeze-dried) powder.PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.PLGF1 Human Recombinant (21-221 a.a.) produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 29kDa.The PLGF-1 Human Recombinant protein is purified by proprietary chromatographic techniques.

Product Specifications

Product Name Alternative

PIGF, PGF, PLGF-1.

Applications

Functional Assay

Purification

Greater than 97.0% as determined by SDS-PAGE.

Components

PLGF-1 protein was lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.It is recommended to reconstitute the lyophilized PLGF1 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions

Lyophilized PLGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Applications Notes

Binds to immobilized rHuVEGFR1 with EC50 less than 6ng/ml.

Amino Acids

AVPPQQWALS AGNGSSEVEV VPFQEVWGRS YCRALERLVD VVSEYPSEVE HMFSPSCVSL LRCTGCCGDE NLHCVPVETA NVTMQLLKIR SGDRPSYVEL TFSQHVRCEC RPLREKMKPE RCGDAVPRR.

Frequently Asked Questions

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