MIF T.Vaginalis

Source: Escherichia Coli.Filtered White lyophilized (freeze-dried) powder.The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. MIF T.Vaginalis Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 1-115) containing 121 amino acids including a 6 a.a C-terminal His tag. The total molecular mass is 13.4kDa (calculated) .

Product Specifications

Product Name Alternative

Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor), Phenylpyruvate Tautomerase, L-Dopachrome Tautomerase, L-Dopachrome Isomerase, GLIF, MMIF, GIF, Macrophage Migration Inhibitory Factor, Glycosylation-Inhibiting Factor, EC 5.3.3.12, EC 5.3.2.1, Macrophage migration inhibitory

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

MIF T.Vaginalis filtered (0.4 μm) and lyophilized from 0.5mg/mL solution in 20 mM Tris buffer and 50 mM NaCl, pH 7.5.It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/mL and let the lyophilized pellet dissolve completely. MIF T.Vaginalis is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage Conditions

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.