EBI3 Macaque

Source: Escherichia Coli.Sterile Filtered White lyophilized (freeze-dried) powder.EBI3 has an induced expression in B lymphocytes in reaction to Epstein-Barr virus infection. EBI3 encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form iIL-27. EBI3 drives rapid clonal expansion of naive cd4 (+) t-cells. EBI3 strongly synergizes with IL-12 to activate IFN-gamma production of naive cd4 (+) t-cells. EBI3 mediates its biologic effects through the cytokine receptor wsx-1/tccr.EBI3 Macaque Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids and having a molecular mass of 23.4kDa. The EBI3 is purified by proprietary chromatographic techniques.

Product Specifications

Product Name Alternative

IL-27B, IL27B, IL 27-B, EBI-3, Interleukin-27 beta, IL-27 subunit beta, Epstein-Barr virus-induced gene 3 protein homolog, EBI3.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid (TFA) .It is recommended to reconstitute the lyophilized EBI3 in sterile 18M Omega -cm H2O at a concentration of 100μg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions

Lyophilized EBI3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EBI3 Macaque should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Amino Acids

MRKGPPAALT LPRVQCRAPR YPIAVDCSWT LPPAPNSTSP VSFIATYRFG MAARGHSWPC LQQTPASTSC TIADVRLFSM APYVLNVTAV HPWGSSSSFV PFIAEHIIKP DPPEGVRLSP LAERQLQVQW EPPRSWPFPE IFSLKYWIRY KRQGAARFHQ VGPIEATSFI LRAVRPRARY CVQVAAQDLT DYGELSDWSL PATTPMSPGK.