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Recombinant Der F1 Protein

Source : E.Coli. The E.Coli derived recombinant protein contains the Dermatophagoides farina Der F1 full length protein (a.a. 1-320) and fused to a 6 His Tag at C-terminus, having a total Mw of 36kDa, pI 5.88. DERF1 is a thiol protease that hydrolyzes proteins, with a preference for Phe or basic residues. DERF1 is a C1 peptidase family member. DERF1 has extensive endopeptidase specificity. DERF1 causes an allergic reaction in humans. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.

Product Specifications

Purification

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Components

60mM NaCl, 50mM Tris-HCl pH 8.0 and 1.2M Urea.

Storage Conditions

Der F1 although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

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