Recombinant Human Heat Shock 22 kDa Protein-8

Source : Escherichia Coli. Recombinant Human Heat Shock Protein 22 kDa Protein-8 is a full-length human HSP22 with an MW of 21604 Dalton produced in E.coli. HSPB8 displays temperature-dependent chaperone activity. HSPB8 acts as a mn (2+) -dependent serine-threonine- specific protein kinase. we are not convinced that this is its true role. Defects in HSPB8 are a cause of distal hereditary motor neuropathy type ii (DHMN2) also known as distal spinal muscular atrophy (DSMA) and spinal muscular atrophy of the charcot-marie-tooth type. it is an autosomal dominant disorder of lower motor neurons characterized by distal muscle weakness.

Product Specifications

Product Name Alternative

HSPB8||H11||HMN2||CMT2L||DHMN2||E2IG1||HMN2A||HSP22||Heat shock protein beta-8||Alpha-crystallin C chain||Small stress protein-like protein HSP22||E2-induced gene 1 protein||Protein kinase H11||CRYAC.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

The HSPB8 protein was lyophilized from a concentrated (1mg/mL) solution containing 20mM Tris-acetate, pH-7.6, 10mM NaCl, 0.1mM EDTA, 0.1mM PMSF, 15mM b-ME.

Storage Conditions

Lyophilized HSPB8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSPB8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized HSPB8 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.