Recombinant Human ZW10 Interacting Kinetochore Protein

Source : Escherichia Coli. ZWINT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 253 amino acids (1-230) and having a molecular mass of 27.9 kDa (Molecular size on SDS-PAGE will appear higher) .ZWINT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. ZW10 Interacting Kinetochore Protein (ZWINT) is involved in kinetochore function. ZWINT is part of the MIS12 complex, which is essential for kinetochore formation and spindle checkpoint activity. ZWINT is localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase. ZWINT interacts with ZW10 (Zeste White 10) and regulates the association between ZW10 and kinetochores. ZWINT gene defects are linked with the pathogenesis of Roberts's syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation.