Recombinant Human Valosin Containing Protein Lysine Methyltransferase

Source : Escherichia Coli. VCPKMT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-229 a.a.) and having a molecular mass of 28.2kDa.VCPKMT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. VCPKMT is a member of the methyltransferase superfamily. VCPKMT is a protein-lysine N-methyltransferase which specifically trimethylates 'Lys-315' of VCP/p97; this alteration reduces VCP ATPase activity.

Product Specifications

Product Name Alternative

Valosin Containing Protein Lysine (K) Methyltransferase||Methyltransferase-Like Protein 21D||VCP Lysine Methyltransferase||Protein-Lysine Methyltransferase METTL21D||Chromosome 14 Open Reading Frame 138||C14orf138||METTL21D||VCP-KMT||EC 2.1.1.- .

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

VCPKMT protein solution (1mg/mL) containing 20mM Phosphate buffer saline (pH 8.0), 1mM DTT and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMADTLES SLEDPLRSFV RVLEKRDGTV LRLQQYSSGG VGCVVWDAAI VLSKYLETPE FSGDGAHALS RRSVLELGSG TGAVGLMAAT LGADVVVTDL EELQDLLKMN INMNKHLVTG SVQAKVLKWG EEIEGFPSPP DFILMADCIY YEESLEPLLK TLKDISGFET CIICCYEQRT MGKNPEIEKK YFELLQLDFD FEKIPLEKHD EEYRSEDIHI IYIRKKKSKF PS