Recombinant Human Small Ubiquitin-Related Modifier 1, His Tag

Source : E.coli. SUMO1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 109 amino acids (1-101) and having a molecular mass of 12.6 kDa.SUMO1 is fused to a 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. SUMO1 is a protein that belongs to the SUMO (small ubiquitin-like modifier) protein family. SUMO1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. Still, unlike ubiquitin which targets proteins for degradation, SUMO1 is involved in a variety of cellular processes, for example nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO1 is not active until the last four amino acids of the carboxy-terminus are cleaved off.

Product Specifications

Product Name Alternative

Small ubiquitin-related modifier 1||SUMO-1||Sentrin||Ubiquitin-like protein SMT3C||SMT3 homolog 3||Ubiquitin-homology domain protein PIC1||Ubiquitin-like protein UBL1||GAP-modifying protein 1||GMP1||SUMO1||SMT3C||SMT3H3||UBL1||PIC1||SMT3||DAP-1||OFC1

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The SUMO1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.15M NaCl and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid multiple freeze-thaw cycles.