Recombinant Human Small Ubiquitin-Related Modifier 1

Source : Escherichia Coli. The active human SUMO-I (the 1-97 animo acid region of the Ubiquitin-like protein SMT3C precursor) . The enzyme contains a single polypeptide band of 11 kDa. The predicted molecular weight of hSOMO I is 11 kDa. The The final fraction of enzyme contains single polypeptide band of approximately 20 kDa on SDS PAGE. SUMO1 is a protein that belongs to the SUMO (small ubiquitin-like modifier) protein family. SUMO1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. Still, unlike ubiquitin which targets proteins for degradation, SUMO1 is involved in a variety of cellular processes, for example nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO1 is not active until the last four amino acids of the carboxy-terminus are cleaved off.

Product Specifications

Product Name Alternative

Small ubiquitin-related modifier 1||SUMO-1||Sentrin||Ubiquitin-like protein SMT3C||SMT3 homolog 3||Ubiquitin-homology domain protein PIC1||Ubiquitin-like protein UBL1||GAP-modifying protein 1||GMP1||SUMO1||SMT3C||SMT3H3||UBL1||PIC1||SMT3||DAP-1||OFC1

Purification

Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

10mM sodium chloride, 100mM imidazole, 0.5mM PMSF, 1mM DTT and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid multiple freeze-thaw cycles.