Recombinant Human SPRY Domain-Containing SOCS Cox Protein 2

Source : Escherichia Coli. SPSB2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 287 amino acids (1-263 a.a) and having a molecular mass of 31.2kDa.SPSB2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. SPRY Domain-Containing SOCS Cox Protein 2 (SPSB2) belongs to a suppressor of cytokine signaling (SOCS) family, and is a member of the subfamily of proteins containing a central SPRY (repeats in splA and RyR) domain and a C-terminal SOCS box. SPSB2 is present in a gene-rich cluster on chromosome 12p13 in the vicinity of the CD4 antigen and triosephosphate isomerase genes. SPSB2 is apparent substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and ensuing proteasomal degradation of target proteins.

Product Specifications

Product Name Alternative

SPRY domain-containing SOCS box protein 2||SSB-2||Gene-rich cluster protein C9||SPSB2||GRCC9||SSB2||GRCC9.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

SPSB2 protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSHMGQTAL AGGSSSTPTP QALYPDLSCP EGLEELLSAP PPDLGAQRRH GWNPKDCSEN IEVKEGGLYF ERRPVAQSTD GARGKRGYSR GLHAWEISWP LEQRGTHAVV GVATALAPLQ TDHYAALLGS NSESWGWDIG RGKLYHQSKG PGAPQYPAGT QGEQLEVPER LLVVLDMEEG TLGYAIGGTY LGPAFRGLKG RTLYPAVSAV WGQCQVRIRY LGERRAEPHS LLHLSRLCVR HNLGDTRLGQ VSALPLPPAM KRYLLYQ.