Recombinant Human Small Nuclear Ribonucleoprotein Polypeptide D3

Source : E.coli. SNRPD3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 146 amino acids (1-126) and having a molecular mass of 16.0 kDa.The SNRPD3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. SNRPD3 is a small nuclear ribonucleoprotein (snRNPs) that contains the spliceosome in eukaryotes. SNRPD3 is essential for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing happens in this locus and two transcript variants encoding the same protein were branded.

Product Specifications

Product Name Alternative

Small nuclear ribonucleoprotein D3 polypeptide 18kDa||Sm-D3||snRNP core protein D3.

Purification

Greater than 85% as determined by SDS-PAGE.

Components

The SNRPD3 solution (0.25mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 0.5M NaCl, 2mM DTT, 0.1mM PMSF and 40% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNKN QGSGAGRGKA AILKAQVAAR GRGRGMGRGN IFQKRR