Recombinant Human Small Nuclear Ribonucleoprotein 25kDa

Source : Escherichia Coli. SNRNP25 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-132 a.a.) and having a molecular mass of 17.4kDa.SNRNP25 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. SNRNP25 is a member of the small nuclear ribonucleoprotein core protein family. SNRNP25 is a component of the U12-type spliceosome which is found in some eukaryotes and removes U12-type introns that are rare and have distinct splice consensus signals. The U12-type spliceosome is comprised of several small nuclear RNAs and associated proteins.

Product Specifications

Product Name Alternative

U11/U12 small nuclear ribonucleoprotein 25 kDa protein||U11/U12 snRNP 25 kDa protein||U11/U12-25K||Minus-99 protein||SNRNP25||C16orf33.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

SNRNP25 protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 2mM DTT and 0.2M NaCl.

Storage Conditions

SNRNP25 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MDVFQEGLAM VVQDPLLCDL PIQVTLEEVN SQIALEYGQA MTVRVCKMDG EVMPVVVVQS ATVLDLKKAI QRYVQLKQER EGGIQHISWS YVWRTYHLTS AGEKLTEDRK KLRDYGIRNR DEVSFIKKLR QK.