Recombinant Human Signaling Threshold Regulating Transmembrane Adaptor 1

Source : Escherichia Coli. SIT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (62-196 a.a.) and having a molecular mass of 16.9kDa (Molecular weight on SDS-PAGE will appear higher) .SIT1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Signaling threshold-regulating transmembrane adapter 1 (SIT1) negatively regulates T-cell antigen receptor (TCR) -mediated signaling in T-cells. SIT1 is involved in positive selection of T-cells. SIT1 is specifically expressed in T- and B-cells. SIT1 is also present in plasma cells but not in germinal center B-cells (at protein level) . SIT1 is expressed in T- and B-cell lymphoma.

Product Specifications

Product Name Alternative

Signaling threshold-regulating transmembrane adapter 1||SHP2-interacting transmembrane adapter protein||Suppression-inducing transmembrane adapter 1||gp30/40||SIT1||SIT||RP11-331F9.5||MGC125908||MGC125909||MGC125910.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

SIT1 protein solution (0.25mg/mL) containing 20mM Tris-HCl buffer (pH8.0) and 20% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MHLSQWTRGR SRSHPGQGRS GESVEEVPLY GNLHYLQTGR LSQDPEPDQQ DPTLGGPARA AEEVMCYTSL QLRPPQGRIP GPGTPVKYSE VVLDSEPKSQ ASGPEPELYA SVCAQTRRAR ASFPDQAYAN SQPAAS.