Recombinant Rat S100 Calcium Binding Protein G

Source : Escherichia Coli. CABP9K (CALB3 or CABP1; mouse, rat, and human 79 aa; chromosome Xp; ~9 kDa) is a cytosolic Ca-binding protein initially found in rat pancreas. It is also expressed in intestine, placenta, uterus and kidney. Its expression is controlled by vitamin D and sex hormone in a tissue specific manner. In keeping with its role in Ca-transport, its expression is highest in duodenal villus enterocytes. It is further shown that CABP9K is only expressed in differentiated enterocytes. CABP9K gene also contains Cdx2-homeoprotein binding sites, and that Cdx2 may play a crucial role in CABP9K transcription.CaBP9K is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Product Specifications

Product Name Alternative

Protein S100-G||S100 calcium-binding protein G||Vitamin D-dependent calcium-binding protein intestinal||CABP||Calbindin-D9k||9 kDa CaBP||Cholecalcin||S100g||Calb3||S100d||Cbpi||MGC72928||Rncalbd9.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The protein was lyophilized from a concentrated solution (1mg/mL) containing 100mM Phosphate buffer, pH 7.3.

Storage Conditions

Lyophilized CABP9K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP9K should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized CABP9K in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.