Recombinant Mouse S100 Calcium Binding Protein A6

Source : Escherichia Coli. S100A6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids (1-89 a.a) and having a molecular mass of 12.2kDa.S100A6 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. S100A6 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized either in the cytoplasm or the nucleus of a wide range of cells. S100 proteins are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. There are at least 13 members in the S100 gene family, which are located as a cluster on chromosome 1q21.S100A6 may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of the S100A6 gene are implicated in melanoma.

Product Specifications

Product Name Alternative

Protein S100-A6||S100 calcium-binding protein A6||Calcyclin||Prolactin receptor-associated protein||5B10||S100a6||Cacy||2A9||PRA.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

S100A6 protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol and 100mM NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.