Recombinant Human S100 Calcium Binding Protein A12

Source : Escherichia Coli. The Recombinant Human S100A12 produced in E.coli has a molecular mass of 11.63kDa containing 101 amino acid residues of the human S100A12 and fused to a 10 a.a. His tag at N-terminus. S100A12 belongs to the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 family members are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A12 may be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.

Product Specifications

Product Name Alternative

Extracellular newly identified RAGE-binding protein||Protein S100-A12||CGRP||Calcium-binding protein in amniotic fluid 1||CAAF1||Calgranulin-C||CAGC||EN-RAGE||Neutrophil S100 protein||S100 calcium-binding protein A12||p6||S100A12||MRP6||ENRAGE.

Components

S100A12 was filtered (0.4μm) and lyophilized in 0.5 mg/mL in 20mM Tris and 50mM NaCl, pH 7.5.

Storage Conditions

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Applications Notes

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.