Recombinant Human RWD Domain Containing 1

Source : Escherichia Coli. RWDD1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 266 amino acids (1-243 a.a.) and having a molecular mass of 30.3kDa (Molecular size on SDS-PAGE will appear higher) .RWDD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. RWD Domain Containing 1 (RWDD1) is a member of the RWDD1/GIR2 family. The RWDD1 protein protects DRG2 (developmentally regulated GTP binding protein 2), from proteolytic degradation. RWD domain refers to 3 signature motifs in proteins: RING finger, WD-repeats, and yeast DEAD (DEXD) -like motif. Current evidence proposes that RWD domain may be required for protein-protein interaction.

Product Specifications

Product Name Alternative

RWD domain-containing protein 1||DRG family-regulatory protein 2||RWDD1||DFRP2||CGI-24||PTD013.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

RWDD1 protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 2mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMTDYGEE QRNELEALES IYPDSFTVLS ENPPSFTITV TSEAGENDET VQTTLKFTYS EKYPDEAPLY EIFSQENLED NDVSDILKLL ALQAEENLGM VMIFTLVTAV QEKLNEIVDQ IKTRREEEKK QKEKEAEEAE KQLFHGTPVT IENFLNWKAK FDAELLEIKK KRMKEEEQAG KNKLSGKQLF ETDHNLDTSD IQFLEDAGNN VEVDESLFQE MDDLELEDDE DDPDYNPADP ESDSAD.