Recombinant Human RNA 3'-Terminal Phosphate Cyclase

Source : Escherichia Coli. RTCA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 389 amino acids (1-366a.a) and having a molecular mass of 41.7kDa. RTCA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. RNA 3'-Terminal Phosphate Cyclase (RTCA) encodes a part of the RNA 3'-phosphate cyclase family. RTCA takes part in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. RTCA is involved in some phases of cellular RNA processing.

Product Specifications

Product Name Alternative

RNA 3'-terminal phosphate cyclase||RPC||RTC1||RTCD1||RNA cyclase||RNA-3'-phosphate cyclase||RNA terminal phosphate cyclase domain-containing protein 1||RTC domain-containing protein 1||RTCA||RPC1.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

RTCA protein solution (0.25mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL.