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Recombinant Human Ras Homolog Gene Family Member V

Source : E.coli. RHOV Human Recombinant produced in E. coli is a single polypeptide chain containing 259 amino acids (1-236) and having a molecular mass of 28.6 kDa.RHOV is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. RHOV belongs to the Rho family and to the small GTPase superfamily. RHOV is a 236 aa protein which regulates the actin cytoskeleton by activating the JNK pathway. RHOV is expressed in placenta, pancreas and fetal brain and operates as a lipid anchor at the cytoplasmic side of the cell membrane.

Product Specifications

Product Name Alternative

Ras homolog family member V||Rho GTPase-like protein ARHV||Wnt-1 responsive Cdc42 homolog 2||WRCH1-related GTPase||GTP-binding protein-like 2||WRCH-2||ARHV||CDC42-like GTPase 2||CHP.

Purification

Greater than 85% as determined by SDS-PAGE.

Components

The RHOV solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 2M Urea, 1mM DTT and 20% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMPPRELS EAEPPPLRAP TPPPRRRSAP PELGIKCVLV GDGAVGKSSL IVSYTCNGYP ARYRPTALDT FSVQVLVDGA PVRIELWDTA GQEDFDRLRS LCYPDTDVFL ACFSVVQPSS FQNITEKWLP EIRTHNPQAP VLLVGTQADL RDDVNVLIQL DQGGREGPVP QPQAQGLAEK IRACCYLECS ALTQKNLKEV FDSAILSAIE HKARLEKKLN AKGVRTLSRC RWKKFFCFV

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